Fluorescence Microscopy Products ...
 
 
 

Quick to perform, fast to report !
It is routinely seen in malaria endemic areas that a major portion of daily working hours is spent on the diagnosis of the disease. Long hours spent in diagnostic work leads to exhaustion and fatigue.

Now, with kapilleryTM you can finish your malaria diagnosis work in a fraction of the time as required by light microscopy!

It takes less than 10 minutes to report diagnosis of malaria using the Kapillery technique. In spite of being highly time efficient; Kapillery boasts of higher sensitivity levels even when compared with thick smear light microscopy!

Very easy to learn and Analyse !
Kapillery has been designed with ease of use in mind. Even a non-clinical staff/lab technician with minimal training for a few days can use Kapillery to perform the tests and interpret the results. Diagnosis of malaria was never so convenient and efficient!! In addition, Florotek BioSystems would be glad to provide assistance in every way for training personnel in use of Kapillery.

 

Ultra Sensitive, Highly Specific !

 

Sensitivity levels provided by Kapillery in diagnosing malaria are far higher than routine light microscopy. Kapillery can report up to 1 parasite per two micro-liters of blood specimen. Unlike other diagnostic tests such as rapid test which provides minimum information about life cycle stages of the parasite; Kapillery is able to differentiate all four species of plasmodium malaria along with detailed information of their respective stages of life cycle. Thus; proving to be highly specific as compared to other diagnostic tests.

Very Economical !
We often see that benefit of modern technology seldom reaches where it is needed the most. This is most often attributed to the high costs involved in undertaking the diagnostic tests. At Florotek Biosystems we aim at providing an advanced technology that is accessible to all. Florotek BioSystems to say the least has achieved the impossible!! No longer is such an advanced diagnostic technology for a privileged few with money. Some very innovative manufacturing practices and honest entrepreneurship have resulted in delivering a technology as advanced as Kapillery at a price that is the lowest in the diagnostic industry today!!


Check out our amazing offers with an authorized Florotek BioSystems dealer in your area today!

 
 
 
COMPOSITION:
The 
KAPILLERYTM  kit contains following for 250 staining.
 

A.O. Tubes

250 units

Tube plugs

250 units

Tube Suspenders

250 units

Forceps

1 units

Immersion Oil

15 ml

Package Insert

 

HOW IT WORKS?

Kapillery tube is a high precision glass haematocrit tube pre-coated with acridine orange fluorescent stain. It is filled with approximately 65 micro-liters of blood specimen from a finger puncture. Kapillery, is then sealed using specially designed tube plug. A precision cylindrical suspender is then inserted inside the open end of the tube. The tube is then centrifuged at 12000 RPM for 5 minutes in a specially designed centrifuge machine. The components of blood then get separated according to their densities to form discreet bands.

Kapillery tube is then placed in the Kapillary holder and examined using a fluorescent microscope. Malaria parasite if present in the specimen absorbs the acridine orange and fluoresce bright green with a characteristic morphology. Parasites are usually observed at RBC and WBC interface.

 

TECHNOLOGY

KAPILLERY TM works on a principle of “Concentration”. It concentrates malaria parasites by centrifugation & highlights them using, “Acridine Orange” fluorescent stain. Concentrating the parasites increases the sensitivity and the fluorescence aids in detecting the glowing parasite with ease.

The key feature of the Kapillery technology is centrifugation. Concentration of red blood cells in a predictable area of the tube makes detection of parasites fast and easy. Red cells concentrate right under the leukocyte and directly above the erythrocyte column. The tube

forces all the surrounding cells in a 40 micron space between its outer and inner circumferences. The parasite takes up acridine orange and can be visualized as a bright spec of light with proper morphology among other non fluorescing red cells. Virtually, all the parasites can be seen by rotating the tube under fluorescent microscope.

FEATURES & BENEFITS

● High sensitivity: Due to large blood sample used and high concentration, this technique has known to record a sensitivity of 1 parasite per 2microlitres of blood.  

● High specificity: Kapillery technique can differentiate all four plasmodium species along with their sexual and asexual parasitemia.  

● Ease of use and Consistency of results: The Kapillery technique is very simple to perform. Parasites can be detected at predictable areas of the tube making detection easier. Factory pre-coated kapillery    tubes provide consistent and uniform staining providing predictable results every single time.  

● Ease of use and Consistency of results: The Kapillery technique is very simple to perform. Parasites can be detected at predictable areas of the tube making detection easier. Factory pre-coated kapillery tubes provide consistent and uniform staining providing predictable results every single time.

●High speed: Requires only 1 minute for reporting a positive sample and maximum of only 3-5 minutes for reporting a negative specimen.  The complete process starting from collecting a blood specimen to   reporting results can be achieved within 10 minutes.

● Minimum training: Kapillery technology can be learned and executed in very little time by even non-clinical staff/ lab technicians. s.  

● Accuracy: Chances of false positive results using Kapillery technique are extremely rare; or almost negligible.  

● Versatility: Kapillery technology can detect microfilaria as well.l.

TRAINING

Processing the KapilleryTM

Process the Kapillery as instructed in KapilleryTM Package Insert.

   
Observing the centrifuged Kapillery blood Specimen.
Due to centrifugation, the plastic suspender suspends itself in between RBC-WBC interface creating discreet bands.
 

   
Placing the tube in a Kapillery holder.

Hold the Kapillery in hand from tube plug end.
Place the tube in Kapillery tube holder as shown in the picture.

 
Focusing the Tube

Place the Kapillery holder on co-axial stage of Floron microscope and put a drop of immersion oil (provided with Kapillery kit) exactly at the interface of RBC-Buffy coat.

Align the centre of Kapillery with the Floron objective and immerse the objective gently in oil drop.

 
 
 

Observing the Negative Specimen

Centrifuged KapillaryTM Showing various cell layers of blood specimen. Note the clear RBC area without any glowing parasites.

Observing the Positive specimen

Upon Centrifugation, parasites occupy pre-determined positions making detection easy and fast. Note various probable positions to locate parasites.

Please note various probable positions to locate parasites.

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P. Falciparum
 

Negative specimen with Granulocytes
overlap in RBC's
(Area of observation: RBC)
P. Falciparum Trophozoites. (Area of observation: RBC)
P. Falciparum Trophozoites. (Area of observation: RBC)
     

 

P. Falciparum Gametocytes (Area of observation: RBC)

NNegative specimen (Area of observation: RBC)

 
 

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P. Vivax
 

P. vivax trophozytes showing irregular cytoplasm
Note bright green nucleus supported with
orange cytoplasm (Area of observation: RBC)

P. vivax schizonts showing black pigments
(Area of observation lymphocytes -monocytes)

 

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Artifact

Microfilaria

   

Absence of specific morphology which disables the
differentiation of nucleus from cytoplasm confirms it as an
artifact. (Area of observation : RBC)

(Ares of observation : Lymphocytes / Monocytes)

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Refer reporting pattern given below

Patient's name :

Investigation requested :

● Malaria Microfilaria

● Result of Screening : Positive Negative

● Species of Malaria : Plasmodium falciparum

● Plasmodium vivax

● Morphological observation

   Trophozoites Schizonts Gametocytes

 
Parasite Index

● I+ = 1 to 10 parasites / Microscopic field.

● II+ = 10 to 50 parasites / Microscopic field.

● III+ = 50 to 100 parasites / Microscopic field.

● IV+ = More than 100 parasites. Microscopic field.

 
Diagnosis of Tuberculosis Bacilli with FLORON

Infection with Mycobacterium tuberculosis remains a major public health problem worldwide. The diagnosis of Tuberculosis is often made on the basis of clinical symptoms, chest X-ray and sputum AFB. The diagnostic tests that are based on immunological principles have yet to overcome the problem of poor sensitivity and specificity. Staining of Mycobacterium tuberculosis with Flour-AFB and observing it under FLORON epi-fluorescent microscope systems offers highly sensitive and specific diagnosis.

Tuberculosis Bacilli stained with Fluor-AFB observed under FLORON 60X oil immersion objective

FAQ'S

At what temp. should I store the KAPILLERY kit ?
KAPILLERY kit can be stored at 15 - 35°c.
 
What type of sample is required to test with KAPILLERY kit?
Whole blood specimen can be collected directly by finger prick or a pre-collected venous blood specimen can be used.
 
How long can I store the KAPILLERY before observation?
It is recommended to observe the tube immediately upon centrifugation however; it is possible to observe these tubes up to 3 days from centrifugation if stored at 15 - 35°c.
 
How do I focus KAPILLERY under microscope?
Place the KAPILLERY in a tube holder. Take a drop of immersion oil (from the bottle provided along with the kit) on buffy coat-RBC interface. Immerse the objective lens slowly into this oil drop without crashing onto the tube. Match the center of the tube with the center of the objective lens’s tip by moving the microscope stage vertically. Firstly, try to focus using the coarse knob followed by gently focusing with the fine knob. When aligned properly, very little blue light can be seen scattered on the tube holder, which indicates proper focusing.
 
How do I identify malaria parasite?
Malaria parasite's DNA/RNA rich structure absorbs acridine orange fluoro-chrome and emits bright green fluorescence when excited by blue light wavelength of around 480nm. RBC's, being non-nucleated appear as a dark brown background. On this dark background, glowing green parasites appear like stars in the sky! Parasite infected RBC's are lighter in weight as compared to healthy RBC's hence; they tend to float and occupy top of the RBC column and some areas in the buffy coat.
In order to locate the parasites all you need to do is observe a few areas like the top layer of the RBC column and the buffy coat. However; it is extremely important to note that, fluorescence is just a primary indicator and gives the probable location of a malaria parasite. Confirmation of malaria parasite is done only after verifying the specific morphology of the parasite. For details on how to identify parasite morphology, please refer to the training section on this website.

LITERATURE

Please click here to download KAPILLERY Literature