HawkEye

● Premium Membrane Assemblies
Ensure crisp, sharp bands, smooth liquid flow, and consistent test performance.

● In-House Reagent Manufacturing
Unlike many kit assemblers, we manufacture our reagents in-house, guaranteeing high concentration, purity, and stability leading to higher sensitivity and lower false results.

● Automated, Controlled Manufacturing
Each step is standardized and validated, ensuring uniform quality across every batch delivered.
The result? A rapid test kit that isn’t just another strip in the box, but a clinically reliable diagnostic tool, trusted by healthcare professionals.

HOW IT WORKS?

Tuberculosis is diagnosed by finding Mycobacterium tuberculosis bacteria in a clinical specimen taken from the patient. While other investigations may strongly suggest tuberculosis, they cannot confirm it.
Wax like coating on the membrane of tuberculosis bacilli has large amount of mycolic acid. This mycolic acid has the tendency to retain the primary stain, like auramine O even after exposure to de-colourizing acid alcohol (hence; the term “AFB” (Acid Fast Bacilli)).
Stained bacteria are then observed using fluorescent microscope to confirm the positive TB test.

TECHNOLOGY

The identification of myco-bacteria with auramine O (fluorescent dye) is due to the affinity of the mycolic acid in the cell wall for the fluoro-chrome.

Therefore; Fluor AFB is fluorescence microscopy based technique.

In fluorescence microscopy, light rays of shorter wavelength pass through a smear stained with fluorescent
dye such as Auramine O. Auromine O has a property of absorbing light rays of shorter wavelength and emitting light rays of longer wavelength. A mercury vapour lamp or a more advanced LED based light source is used as a source of light. Either with LED of predefined wavelength or by means of suitable filter, only light rays of shorter wavelength are allowed to emerge. These rays are then used for microscopy.